Here’s a list of my fav’s so far:
The whole Questionable Ethics chapter, especially the ending battle, the hardest, but by far my favorite HECU battle so far. Also releasing the Agrunts and watching them take down the HECU.
The first outside area of We’ve got Hostiles - it was such an “Ohhhh Shit” moment when you step out side to try and flank the soldiers, and then you see a shitload of mortars launch in the distance and you know if you stay there you are gonna die.
The gargantua in power up, when you sit in the doorway thinking your safe, and then he KNEELS DOWN and sticks his hand in to burn you 
The falling elevator in Unforeseen Consequences, the one that YOU are in. That was a really nice touch, adding that whole new sequence and letting us fall to what we think will cause at least some injury.
The emergence from the tunnel to see the entire 3D REAL GEOMETRY SKYBOX of Surface Tension. That thing was beautiful and mind-blowing.
The helicopter showdown, it was hard but satisfying to take down that heli, especially since the damn thing didn’t even give me a chance to look around when I was on the dam.
The Garbage Compactor scene. I am a huge sucker for traps, and this one takes the cake. I actually got squished the first few times on purpose just to watch this damn thing crush all those boxes.
And yes, the Hornet Gun